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- E-ISSN 2288-8985
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Analysis of calmodulin binding property of IQ motifs of IQGAP1
Analytical Science and Technology / Analytical Science and Technology, (P)1225-0163; (E)2288-8985
2011, v.24 no.6, pp.527-532
https://doi.org/10.5806/AST.2011.24.6.527
(2011). Analysis of calmodulin binding property of IQ motifs of IQGAP1. Analytical Science and Technology, 24(6), 527-532, https://doi.org/10.5806/AST.2011.24.6.527
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Abstract
IQ motif-containing GTPase-activating protein 1 (IQGAP1), which is a well-known Ca^(2+)-independent calmodulin (CaM) binding protein, is involved in various cellular functions such as cell proliferation and cell migration. IQGAP1 has four repeated IQ motifs, which are crucial for CaM binding. It has been shown that all four IQ motifs of IQGAP1 can bind to Ca^(2+)/CaM, while the third and fourth IQ motifs of IQGAP1 can bind to apoCaM. However, it has not been clear whether the CaM binding of IQ motifs of IQGAP1 was mediated directly or indirectly. In this study, we examined whether the binding between CaM and each IQ motif of IQGAP1 was direct in vitro. As a result, we found that IQ1 motif has a weak Ca^(2+)-dependent CaM binding. In contrast, IQ3 has a Ca^(2+)-dependent CaM binding. All other motifs have no significant CaM binding. We also found that IQ(2.7-3) and IQ(3.5-4.4) have CaM binding capacity. This finding indicates that IQ motifs of IQGAP1 plays a dynamic role via different motif interactions with Ca^(2+)/CaM or proCaM.
- keywords
- IQGAP1, IQ motif, calmodulin
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