- P-ISSN 1225-0163
- E-ISSN 2288-8985
한외여과법을 이용하여 봉독의 알러지 원인성분인 PLA2를 제거하기 위하여 압력, 농도, 분자크기의 영향을 조사하였다. 봉독의 주요성분 분자량을 바탕으로 한외여과막의 투과크기를 선정하고 농도와 압력을 달리하였다. 그 결과, melittin과 apamin 함량은 유지되면서 PLA2를 제거하는 최적조건(1 mg/mL,20 psi, 10,000 dalton)을 찾았으며, 이를 HPLC와 SDS-PAGE로 확인하였다
The effects of concentration, pressure, and molecular sige on removing allergenic substance(phospholipase A2) in bee venom by ultrafiltration were investigated. The membrane pore sizes wereselected based on the molecular weight of the main compounds. The conditions of concentration andpressure were selected randomly. As results, we obtained the optimum condition (1 mg/mL, 20 psi, 10,000dalton) for removing PLA2 at constant concentration of melittin and apamin and confirmed the separationresults by HPLC and SDS-PAGE.
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