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Effect of concentration, pressure, and cut-off size on removing phospholipase A2 in bee venom by ultrafiltration
Analytical Science and Technology / Analytical Science and Technology, (P)1225-0163; (E)2288-8985
2014, v.27 no.6, pp.277-283
https://doi.org/10.5806/AST.2014.27.5.277
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(2014). Effect of concentration, pressure, and cut-off size on removing phospholipase A2 in bee venom by ultrafiltration. Analytical Science and Technology, 27(6), 277-283, https://doi.org/10.5806/AST.2014.27.5.277
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Abstract
The effects of concentration, pressure, and molecular sige on removing allergenic substance(phospholipase A2) in bee venom by ultrafiltration were investigated. The membrane pore sizes wereselected based on the molecular weight of the main compounds. The conditions of concentration andpressure were selected randomly. As results, we obtained the optimum condition (1 mg/mL, 20 psi, 10,000dalton) for removing PLA2 at constant concentration of melittin and apamin and confirmed the separationresults by HPLC and SDS-PAGE.
- keywords
- honey, bee venom, phospholipase <TEX>$A_2$</TEX>, ultrafiltration
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