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Tissue-Based Amperometric Biosensor for Determination of L-Ascorbic Acid in Organic Media
Analytical Science and Technology / Analytical Science and Technology, (P)1225-0163; (E)2288-8985
1994, v.7 no.2, pp.149-153
Kwon,
H., &
Lee,
C.
(1994). Tissue-Based Amperometric Biosensor for Determination of L-Ascorbic Acid in Organic Media. Analytical Science and Technology, 7(2), 149-153.
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Abstract
An amperometric sensor for L-ascorbic acid(AA) in methanol media has been made by immobilizing squash-tissues on a graphite rod disk. A detection limit of the electrode was <TEX>$2{\times}10^{-6}M$</TEX> L-ascorbic acid. In comparison with an isolated enzyme based ascorbate oxidase(AO) electrode, the plant-tissue electrode offered high biocatalytic stability and activity and extremely low cost. The electrode has a useful lifetime of 1 week.
- keywords
- Amperometric Biosensor, L-Ascorbic Acid(AA), Ascorbate oxidase(AO), Squash, Graphite rod disk
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