• P-ISSN1225-0163
  • E-ISSN2288-8985
  • SCOPUS, ESCI, KCI

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  • P-ISSN 1225-0163
  • E-ISSN 2288-8985

Tissue-Based Amperometric Biosensor for Determination of L-Ascorbic Acid in Organic Media

Analytical Science and Technology / Analytical Science and Technology, (P)1225-0163; (E)2288-8985
1994, v.7 no.2, pp.149-153
Kwon, Hyo-Shik
Lee, Cheal-Gyu
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Abstract

An amperometric sensor for L-ascorbic acid(AA) in methanol media has been made by immobilizing squash-tissues on a graphite rod disk. A detection limit of the electrode was <TEX>$2{\times}10^{-6}M$</TEX> L-ascorbic acid. In comparison with an isolated enzyme based ascorbate oxidase(AO) electrode, the plant-tissue electrode offered high biocatalytic stability and activity and extremely low cost. The electrode has a useful lifetime of 1 week.

keywords
Amperometric Biosensor, L-Ascorbic Acid(AA), Ascorbate oxidase(AO), Squash, Graphite rod disk


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