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Determination of Sodium Chondroitin Sulfate by Enzymatic Digestion and HPLC
Analytical Science and Technology / Analytical Science and Technology, (P)1225-0163; (E)2288-8985
1994, v.7 no.2, pp.245-251
Kang,
S., Shin,
H., Chang,
S., &
Yoon,
H.
(1994). Determination of Sodium Chondroitin Sulfate by Enzymatic Digestion and HPLC. Analytical Science and Technology, 7(2), 245-251.
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Abstract
In order to determine sodium chondroitin sulfate in the mixture, chondroitinase ABC was used for enzymatic reaction. The procedure was rapid, simple, quantitative and the HPLC analysis of <TEX>${\Delta}Di-6S$</TEX>(2-actamido-2-deoxy-3-0-(<TEX>${\beta}$</TEX>-D-gluco-4-enepyranosyluronic acid)-6-0-sulfo-D-galactose) in the sodium chondroitin sulfate was obtained. The absorbance was measured at 230nm and detection limit was <TEX>$1{\mu}g/ml$</TEX>. When we applied this method to the drugs(capsule, opthalmic solution), it gave the mean contents of <TEX>$100.01{\pm}1.58%$</TEX> and <TEX>$99.89{\pm}1.80%$</TEX> respectively.
- keywords
- Sodium chondroitin sulfate, chondroitinase ABC, HPLC, enzymatic reaction
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