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- E-ISSN 2288-8985
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Determination of <TEX>$Ca^{2+}$</TEX> by Fiber Optic Fluorosensor Based on the Conformational Change of the Protein Calmodulin
Analytical Science and Technology / Analytical Science and Technology, (P)1225-0163; (E)2288-8985
1995, v.8 no.3, pp.221-227
Ri,
C., &
Yang,
S.
T.
(1995). Determination of <TEX>$Ca^{2+}$</TEX> by Fiber Optic Fluorosensor Based on the Conformational Change of the Protein Calmodulin. Analytical Science and Technology, 8(3), 221-227.
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Abstract
The fiber optic fluorosensor that shows a specific selectivity for calcium ion is studied. This sensor employs protein Calmodulin(CaM) which forms a fluorescent chelate with <TEX>$Ca^{2+}$</TEX>. A dialysis membrane is used to entrap a fluorescein isothiocyanate-labeled CaM solution at the common end of a bifurcated fiber optic bundle. The sensing mechanism of this sensor is based on the shifts in the fluorescence spectrum of metal-calmodulin complexes which FCaM forms a chelate with <TEX>$Ca^{2+}$</TEX>. Upon binding with <TEX>$Ca^{2+}$</TEX>, CaM undergoes a conformational change which induces a change in the fluorescence of FCaM. This change in fluorescence signal which is measured by photomultiflier tube is related to the concentration of <TEX>$Ca^{2+}$</TEX> for calibration curve. Detection limit for <TEX>$Ca^{2+}$</TEX> and the interference effects by <TEX>$Mg^{2+}$</TEX>, <TEX>$Eu^{3+}$</TEX> and <TEX>$La^{3+}$</TEX> for this sensor are studied. Response time and life time for this fluorosensor are also investigated.
- keywords
- Fiber Optic Sensor, Fluorescence, Calcium ion, Calmodulin
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