- P-ISSN 1225-0163
- E-ISSN 2288-8985
- P-ISSN1225-0163
- E-ISSN2288-8985
- SCOPUS, ESCI, KCI
Article Detail
Home > Article Detail
Two-enzyme coupled fluorometric assay of urinary dipeptidase
Analytical Science and Technology / Analytical Science and Technology, (P)1225-0163; (E)2288-8985
1995, v.8 no.3, pp.359-364
Park,
H. S., &
We,
J.
S.
(1995). Two-enzyme coupled fluorometric assay of urinary dipeptidase. Analytical Science and Technology, 8(3), 359-364.
- Downloaded
- Viewed
Abstract
Urinary dipeptidase(Udpase) was assayed by fluorometric analysis of NADH which was produced from an indicator enzyme, L-alanine dehydrogenase. The reaction mixture was consisted of a dipeptide(L-ala-L-ala), <TEX>${\beta}-NAD^+$</TEX>, L-alanine dehydrogenase in 12.5 mM sodium carbonate buffer, pH 9.0, and urinary dipeptidase which initiated the reaction. The fluorescence intensity of NADH was measured as a function of time with the excitation wavelength at 340nm and emission at 460nm. Comparison of this fluorometric method with the conventional spectrophotometric method utilizing glycyldehydrophenylalanine(Gdp) as substrate provided the correlation coefficient of 0.996 and increased the sensitivity more than ten times.
- keywords
- urinary dipeptidase(Udpase), fluorometric assay, L-alanine dehydrogenase, glycyldehydrophenylalanine (Gdp)
- Downloaded
- Viewed
- 0KCI Citations
- 0WOS Citations
Other articles from this issue
- Determination of <TEX>$Ca^{2+}$</TEX> by Fiber Optic Fluorosensor Based on the Conformational Change of the Protein Calmodulin
- Determination of free amino acids in the plasma samples of normal subjects and schizophrenic subjects in Korea by HPLC
- Simultaneous Analysis of Pesticide Priority Pollutants in Soil
- The study on the spectroscopic and electrical properties of Azomethine compounds
- Separation of Hg(II) by using the poly-NTOE(1, 12-diaza-3, 4:9, 10-dibenzo-5, 8-dioxacyclopentadeca-1, 12-ylene-2, 7-dihydroxyoctamethylene) in Liquid Chromatography
- Development of Schiff Base Column and Glow Discharge Detector for HPLC : Preliminary Study I
- Preparation and analysis of lyophilized whole blood as external quality control materials for Pb and Cd determination by graphite furnace atomic absorption spectrometry
- Effect of Oxidant Concentration and Reflux Time on COD Value by Alkaline Permanganate Method
- Determination of Uranium using 1-(2-Pyridylazo)-2-naphthol by Adsorptive Stripping Voltammetry
- Study of the Stability of Brass Coated on Steel Cords with pH and Applied Constant Potential Changes in Aqueous Solutions by AC Impedance Measurements
- Separation of Cu(II) from Metal Mixture Solution Using a Hexadecyl <TEX>$NtnOenH_4$</TEX>-Octadecylsilanized Silicas(ODS) in Liquid Chromatography
- The Complex Formation of Fe(III)-Salicylic acid
- Surface-Enhanced Raman Scattering Spectroscopic Identification of Genotoxic Nucleobase Adducts
- Studies on Partition and Extraction Equilibria of Metal-Dithiocarbamate Complexes(II). Metal Complexes of Ammonium Pyrrolidine Dithiocarbamate
- Automatic On-line BOD Measurement System Using A Microbial Membrane Electrode
- Analysis of magnetic properties for the melt-spun <TEX>$Nd_{14}Fe_{80}B_6$</TEX> ribbon alloy
- A Study of Oxidation Behavior on the Surface of Nd-Fe-B Ribbon Alloy by X-ray Photoelectron Spectroscopy
- Two-enzyme coupled fluorometric assay of urinary dipeptidase
- Liver tissue sensor for the determination of dopamine
- Analysis of organic germanium, Ge-132
- Analysis of Indole-3-acetic acid from bean sprouts by GC/MSD
- The electrochemical desorption of an eicosanethiol monolayer by electrochemical quartz crystal microbalance
- Determination of Gold by Flame Atomic Absorption Spectrophotometry using a New Chelating Agent
- Separation of Th(IV) and U(VI) Using Arsenazo I-XAD-16 Chelating Resin
- more
Recommanded Articles
This website recommended the use the Chrome browser for journal website.