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Analysis of Indole-3-acetic acid from bean sprouts by GC/MSD
Analytical Science and Technology / Analytical Science and Technology, (P)1225-0163; (E)2288-8985
1995, v.8 no.3, pp.375-381
Lee,
J., Han,
I., Lee,
S., Hawer,
W., &
Yeo,
I.
(1995). Analysis of Indole-3-acetic acid from bean sprouts by GC/MSD. Analytical Science and Technology, 8(3), 375-381.
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Abstract
A gas chromatographic analytical method for IAA was developed. IAA was extracted with 80% acetone & methanol. Sample was macerated with 80% acetone & methanol to extract IAA. After filtration, the solvent was removed and residual IAA was esterified with 14% <TEX>$BF_3$</TEX>/MeOH. The IAA esters can be easily separated with intermediate polar column.
- keywords
- Indole-3-acetic acid (IAA), MethyI ester, Bean sprouts, GC/MSD
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