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Article Detail
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Liver tissue sensor for the determination of dopamine
Analytical Science and Technology / Analytical Science and Technology, (P)1225-0163; (E)2288-8985
1995, v.8 no.3, pp.365-370
Hur,
M., Kim,
K., Kim,
Y., Paek,
K., Choi,
H., &
Ahn,
M.
(1995). Liver tissue sensor for the determination of dopamine. Analytical Science and Technology, 8(3), 365-370.
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Abstract
A tissue biosensor for the measurement of dopamine has been constructed by immobilizing the slice of Sprague-Dawley rat liver on <TEX>$NH_3$</TEX>-sensing electrode. To overcome the defect of tissue sensors, the maximal velocity of response curve was measured and applied to the Lineweaver-Burk equation instead of the Nernst equation. And then compared the results with those obtained from Nernst equation. When we obtained calibration curves from Nernst equation, there were variances on the slope and the linear range. But from Lineweaver-Burk equation, the scale of variance was small. Response time was reduced from 7~12 minutes to 2~3 minutes.
- keywords
- Tissue biosensor, dopamine, Lineweaver-Burk equation
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