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Surface-Enhanced Raman Scattering Spectroscopic Identification of Genotoxic Nucleobase Adducts
Analytical Science and Technology / Analytical Science and Technology, (P)1225-0163; (E)2288-8985
1995, v.8 no.3, pp.313-319
Kim,
J.
(1995). Surface-Enhanced Raman Scattering Spectroscopic Identification of Genotoxic Nucleobase Adducts. Analytical Science and Technology, 8(3), 313-319.
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Abstract
Surface-enhanced Raman scattering(SERS) spectroscopy was employed to analyze the genotoxic nucleobase adducts of benzo[a]pyrene(BP) formed through one-electron oxidation pathway. SERS spectroscopy provided sufficient resolution to distinguish if BP intermediate was bound to different nucleobases(e. g. adenine or guanine). Furthermore, SERS specroscopy was also able to detect the difference in the binding position of the adduct to the various sites of the nucleobase. The linearity of the calibration curve for N7Ade-BP ranged from 20 picogram to 800 nanogram per microliter and the detection limit under the current conditions was determined 20 picogram per microliter in a solution volume of 20 microliter.
- keywords
- Surface-Enhanced Raman Scattering, Genotoxic nucleobase, Benzo[a]pyrene, Analytical Spectroscopy
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