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Determination of free amino acids in the plasma samples of normal subjects and schizophrenic subjects in Korea by HPLC
Analytical Science and Technology / Analytical Science and Technology, (P)1225-0163; (E)2288-8985
1995, v.8 no.3, pp.229-236
Park,
S. S., Park,
S., Pyo,
H. S., Park,
J., Park,
T. K., &
Shin,
Y.
M.
(1995). Determination of free amino acids in the plasma samples of normal subjects and schizophrenic subjects in Korea by HPLC. Analytical Science and Technology, 8(3), 229-236.
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Abstract
Multiple-step gradient method was used for the analysis of free amino acids in physiological fluids by high-performance liquid chromatography with diode array detector on the Amino Quant <TEX>$C_{18}$</TEX> column under the condition of pH 7.2 of buffer solutions. Plasma samples of normal Korean people and abnormal Korean people who have schizophrenia were subjected to derivatization with o-phthalaldehyde in the presence of 3-mercaptopropionic acid. Quantitative analysis of amino acids in physiological fluids by internal standard method gave highly reproducible results within a relative standard deviation of less than 2~6%. And amino acids amounts of physiological fluids of Korean people gave some different results from those of foreigners. There was large differences in tyrosine amount between normal and abnormal man.
- keywords
- free amino acids, plasma, HPLC, schizophrenic subjects
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