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ISSN : 1225-3480
본 연구는 납에 노출된 바지락, Ruditapes philippinarum의 생물학적 반응을 알아보고자 하였다. 실험기간은 4주였으며, 실험구는 대조구 1개와 납 노출구 3개 (0.25, 0.50 and1.00 mg/l) 였다. 실험 결과 납은 바지락의 생존율과 산소 소비율의 저하 및 기관계의 조직학적 변성을 유도하는 것이 확인되었다. 납 노출구에서 산소소비율은 25-72% 감소하였다. 기관계의 조직학적 분석 결과, 외투막 상피층과 결합조직층의 변성, 아가미 점액세포의 증가와 상피세포의 괴사, 발에서는 상피층의 붕괴, 점액세포의 감소 및 혈림프동의 확장과 결합조직층의 변성을 나타냈다. 소화맹낭의 소화선세관에서는 호염기성세포와 상피세포의 위축 및 농도 의존적으로 lipofuscin의축적이 확인되었다.
This study was conducted to find out biological response of Manila clam, Ruditapes philippinarum exposed to lead(Pb). Experimental period was four weeks. Experimental groups were composed of one control condition and threelead exposure conditions (0.25, 0.50 and 1.00 mg/l). The results of the study confirmed that lead induces reductionof survival rate and oxygen consumption rate and histopathology of organ structure of the bivalve. Oxygenconsumption rate was observed exposure groups lower than control decline by 25%-72%. Histological analysis oforgan system illustrated expansion of hemolymph sinus, disappearance of epidermal layer and degeneration ofconnective tissue layer of the mantle. Also, histological degenerations as epithelial necrosis and hyperplasia ofmucous cells are recognized in the gill and it was observed expansion of hemolymph sinus, disruption of epitheliallayer, decrease of mucous cell and degeneration of connective tissue layer in the foot. In the digestivediverticulum, it was showed atrophy of basophilic cell and degeneration of epithelial cell in the digestive tubules,and as the concentration of lead increased the accumulation of lipofuscin increased.
Andhale, A.V., Bhosale, P.A. and Zambare, S.P. (2011) Histopathological study of nickel induced alterations in the fresh water bivalve, Lammellidens marginalis. Journal of Experiment. Sciences, 2: 1-3.
Beninger, P.G., Le Pennec, M. and Salaun, M. (1988) New observations of the gills of Placopecten magellanicus (Mollusca: Bivalvia), and implications for nutrition. Marine Biology, 98: 61-70.
Brown, B.E. and Newell, R.C. (1972) The effect of copper and zinc on the metabolism of the mussel Mytilus edulis. Marine Biology, 16: 108-118.
Byrne, P.A. and O'Halloran, J. (1999) Aspects of assaying sediment toxicity in Irish estuarine ecosystems. Marine Pollution. Bulletin, 39: 97-105.
Cheung, S.G. and Cheung, R.Y. (1995) Effects of heavy metals on oxygen consumption and ammonia excretion in green-lipped mussels (Perna viridis). Marine Pollution. Bulletin, 31: 381-386.
Chin, P., Lee, J.A., Shin, Y.K. and Lee, J.S. (1999) Environmental tolerance for pollutants in Littorina brevicula (Philippi). 2. The growth, metabolism and histological changes exposed to TBTCl and heavy metals in Littorina brevicula. Journal of Korean. Fisheries Society, 32: 593-600. [in Korean]
Cooper, N.L., Bidwell, J.R. and Kumar, A. (2009) Toxicity of copper, lead, and zinc mixtures to Ceriodaphnia dubia and Daphnia carinata. Ecotoxicology Environmental Safety, 72: 1523–1528.
Da Ros, L., Nasci, C., Marigómez, I. and Soto, M. (2000) Biomarkers and trace metals in the digestive gland of indigenous and transplanted mussels, Mytilus galloprovincialis, in Venice Lagoon, Italy. Marine Environmental Research, 50: 417–423.
Depledge, M.H. and Hopkin, S.P. (1995) Methods to assess effects on brackish, estuarine and near-coastal water organisms. In: Methods to assess the effects of chemicals on ecosystems. (ed. by Linthurst, R.A., Bourdeau, P. and Tardiff, R.G.). pp. 125-149. Wiley, Chichester, UK.
Domouhtsidou, G.P. and Dimitriadis, V.K. (2001) Lysosomal and lipid alterations in the digestive gland of mussels, Mytilus galloprovincialis (L.) as biomarkers of environmental stress. Environmental Pollution, 115: 123-137.
Eble, A.F. (2001) Anatomy and histology of Mercenaria mercenaria. In: Biology of the hard clam. (ed. by Kraeuter, J.N., Castagna, M.). pp. 117-220. Elsevier, New York.
Fichet, D., Radenac, G. and Miramand, P. (1998) Experimental studies of impacts of harbour sediments resuspension to marine invertebrates larvae: Bioavailability of Cd, Cu, Pb and Zn and toxicity. Marine Pollution Bulletin, 36: 509-518.
Gregory, M.A., George, R.C., Marshall, D.J., Anandraj, A. and Mcclurg, T.P. (1999) The effects of mercury exposure on the surface morphology of gill filament in Perna perna (Mollusca: Bivalvia). Marine Pollution Bulletin, 39: 116-121.
Hebel, D.K., Jones, M.B. and Depledge, M.H. (1997) Responses of crustaceans to contaminant exposure: a holistic approach. Estuarine, Coastal and Shelf Science, 44: 177-184.
Huggett, R.J., Kimerle, R.A., Mehrle, P.M. Jr and Bergman, H.L. (1992) Biomarkers: biochemical, physiological, and histological markers of anthropogenic stress. pp. 1-347. Lewis Publishers, Boca Raton, FL.
Husmann, G., Abele, D., Monien, D., Monien, P., Kriews, M. and Philipp, E.E.R. (2012) The influence of sedimentation on metal accumulation and cellular oxidative stress markers in the Antarctic bivalve Laternula elliptica. Coastal and Shelf Science, 111: 48-59.
Ju, S.M., Lee, J.W., Jin, Y.G., Yu, J. and Lee, J.S. (2006) Effect of zinc bioaccumulation on survival rate,activity, growth and organ structure of the equilateral venus, Gomphina veneriformis (Bivalvia: Veneridae). Journal of Environmental Toxicology, 21: 115-126. [in Korean]
Kang, J.C., Kim, H.Y. and Chin, P. (1997) Toxicity of cooper, cadmium and chromium on survival, growth and oxygen consumption of the mysid, Neomysis awatschensis. Journal of Korean. Fisheries Society, 30: 874-881. [in Korean]
Kim, S.Y. and Lee, T.Y. (1988) The effects of pollutants effluent from a steam-power plant on coastal bivalves. Ocean Research, 10: 47-56. [in Korean]
Krishnakumar, P.K., Asokan, P.K. and Pillai, V.K. (1990) Physiological and cellular responses to copper and mercury in the green mussel Perna viridis (Linnaeus). Aquatic Toxicology, 18: 163-173.
Lee, J.S. and Park, J.J. (2007) Risk assessment of nonylphenol using the sex ratio, sexual maturation, intersex and lipofuscin accumulation of the equilateral venus, Gomphina veneriformis (Bivalvia: Veneridae). Journal of Korean. Fisheries Society, 40: 16-23. [in Korean]
Marin, M.G., Boscolo, R., Cella, A., Degetto, S. and Ros, L.D. (2006) Field validation of autometallographical black silver deposit (BSD) extent in three bivalve species from the Lagoon of Venice, Italy (Mytilus galloprovincialis, Tapes philippinarum, Scapharca inaequivalvis) for metal bioavailability assessment. Science of Total Environment, 371: 156-167.
Moore, M.N. (1988) Cytochemical responses of the lysosomal system and NADPH-ferrihemoprotein reductase in molluscan digestive cells to environmental and experimental exposure to xenobiotics. Maine. Ecology Progress Series, 46: 81-89.
Moore, M.N. and Allen, J.I. (2002) A computational model of the digestive gland epithelial cell of the marine mussel and its simulated responses to oil-derived aromatic hydrocarbons. Marine Environmental Research, 54: 579-584.
Morrison, C.M. (1993) Histology and cell ultrastructure of the mantle and mantle lobes of the eastern oyster, Crassostrea virginica, Gmelin: A summary atlas. American Malacological Bulletin, 10: 1-24.
NRC (National Research Council). (1983) Risk assessment in the federal government: managing the process. pp. 1-192. National Academy Press, Washington, D.C.
Otludil, B., Cengiz, E.I., Yildirim, M.Z., Unver, O. and Unlu, E. (2004) The effects of endosulfan on the great ramshorn snail, Planorbariua corneus (Gastropoda, Pulmonata): a histopathological study. Chemosphere, 56: 707-716.
Park, J.J. and Lee, J.S. (2008) Tributyltin chloride (TBTCI) toxicity on the growth and mantle structure of the equilateral venus, Gomphina veneriformis (Bivalvia: Veneridae). The Korean Journal of Malacology, 24: 229-241. [in Korean]
Park, J.S. and Lee, J.S. (2011) Change of reproductive and histological biomarkers of Ruditapes philippinarum (Bivalvia: Veneridae) exposed to nonylphenol. The Korean Journal of Malacology 27: 181-190. [in Korean]
Reeve, M.R., Gamble, J.C. and Walter, M.C. (1977) Experimental observation on the effects of cooper on copepod and other zooplankton: controlled ecosystem pollution experiment. Bulletin of Marine Science, 27: 92-104.
Regoli, F. (1992) Lysosomal responses as a sensitive stress index in biomonitoring heavy metal pollution. Maine. Ecology Progress Series, 84: 63-69.
Schintu, M., Durante, L., Maccioni, A., Meloni, P., Degetto, S., and Contu, A. (2008) Measurement of environmental trace-metal levels in mediterranean coastal areas with transplanted mussels and DGT techniques. Marine Pollution Bulletin, 57: 832-837.
Shin, Y.K., Kim, B.H. Oh, B.S., Jung, C.G., Sohn, S.G. and Lee, J.S. (2006) Physiological responses of the ark shell, Scapharca broughtonii (Bivalvia: Arcidae) to decrease in salinity. Journal of Fisheries Science and Technology, 9: 153-159. [in Korean]
Soto, M., Cajaraville, M.P. and Marigómez, I. (1996) Tissue and cell distribution of copper, zinc and cadmium in the mussel, Mytilus galloprovincialis, determined by autometallography. Tissue and Cell, 28: 557-568.
Sunila, I. (1988) Acute histological responses of the gill of the mussel, Mytilus edulis, to exposure by environmental pollutants. Journal of Invertebrate Pathology, 52: 137-141.
Vasanthi, L.A., Revathi, P., Arulvasu, C. and Munuswamy, N. (2012) Biomarkers of metal toxicity and histology of Perna viridis from Ennore estuary, Chennai, south east coast of India. Ecotoxicology Environmental Safety, 84: 92–98.
Viarengo, A. (1985) Biochemical effects of trace metals. Marine Pollution Bulletin, 16: 153-158.
Viarengo, A., Canesi, L., Pertica, M., Poli, G., Moore, M.N. and Orunesu, M. (1990) Heavy metal effects on lipid peroxidation in the tissues of Mytilus galloprovincialis Lam. Comparative Biochemistry and Physiology - part C, 97: 37-42.
Vijayavel, K., Gopalakrishnan, S. and Balasubramanian, M.P. (2007) Sublethal effect of silver and chromium in the green mussel Perna viridis with reference to alterations in oxygen uptake, filtration rate and membrane bound ATPase system as biomarkers. Chemosphere, 69: 979–986.
Watling, H.R. and Watling, R.J. (1982) Comparative effects of metals on the filtering rate of the brown mussel, Perna perna. Bulletin of Environmental Contamination and Toxicology, 29: 651-657.
Zorita, I., Ortiz-Zarragoitia, M., Soto, M., Cajaraville, M.P. (2006). Biomarkers in mussels from a copper site gradient (Visnes, Norway): an integrated biochemical, histochemical and histological study. Aquatic Toxicology, 78: S109-S116.