- P-ISSN 1225-0163
- E-ISSN 2288-8985
글루코스 옥시다아(GOx)제 고정화 바이오센서를 두 가지 방법으로 제조 하였다. 첫 번째 방법은 폴리(maleic anhydride) 그래프트 탄소나노튜브(PMAn-g-MWCNT) 전극에 감마선 조사법으로 제조 된 Au 나노입자를 물리적으로 흡착시킨 후, GOx을 고정화 시켜 바이오센서를 제조한 경우이고, 다른 하나는 PMAn-g-MWCNT 전극에서 Au 이온을 전기화학적으로 환원시켜 Au 나노입자를 코팅 시키고, 그 위에 GOx을 고정화 시켜 바이오센서를 제조 한 경우이두. 제조된 바이오센서에 대해 효율 평가를 수행 하였는데, 물리적 흡착법으로 제조된 전극의 경우 검출 범위는 <TEX>$30\;{\mu}M\sim100\;{\mu}M$</TEX>이었으며, 검출한계는 <TEX>$15\;{\mu}M$</TEX>이었다. 또한 ascorbic acid와 uric acid에 대한 검출한계는 7.6%이었다. 물리적으로 Au 나노입자가 흡착된 전극의 경우가 글루코스 측정에 매우 우수한 전극임을 확인 하였다.
Glucose oxidase (GODox) immobilized biosensor was fabricated by two methods. In one of the methods, gold nanoparticles (Au-NPs) prepared by γ-irradiation were loaded into the poly(maleic anhydride)-grafted multi-walled carbon nanotube, PMAn-g-MWCNT electrode via physical entrapment. In the other method,the Au-NPs were prepared by electrochemical reduction of Au ions on the surface of PMAn-g-MWCNT electrode and then GODox was immobilized into the Au-NPs. The GODox immobilized biosensors were tested for electrocatalytic activities to sense glucose. The sensing range of the biosensor based on the Au-NPs physically modified PMAn-g-MWCNT electrode was from 30 μM to 100 μM for the glucose concentration, and the detection limit was 15 μM. Interferences of ascorbic acid and uric acid were below 7.6%. The physically Au deposited PMAn-g-MWCNT paste electrodes appear to be good sensor in detecting glucose.
1. Z. Zhuang, X. Su, H. Yuan, Q. Sun, D. Xiao, and M.M.F. Choi, Analyst, 133, 126-132(2008).
2. F. Kurniawan, V. Tsakova, and V.M. Mirsky, Electroanalysis, 18, 1937-1942(2006).
3. L. Q. Rong, C. Yang, Q. Y. Qian, and X. H. Xia, Talanta, 72, 819-824(2007).
4. J. S. Ye, Y. Wen, W. D. Zhang, L. M. Gan, G. Q. Xu, and F. S. Sheu, Electrochem. Comm., 6, 66-70(2004).
5. C. K. Tan, K. P. Loh, and T. T. L. John, Analyst, 133, 448-451(2008).
6. S. Chakraborty and C. R. Raj, J. Electroanal. Chem., 609, 155-162(2007).
7. K. B. Male, S. Hrapovic, and J. H. T. Luong, Analyst, 132, 1254-1261(2007).
8. A. Arvinte, A. M. Sesay, V. Virtanen, and C. Bala, Electroanalysis, 20, 2355-2362(2008).
9. Y. Liu, M. K. Wang, F. Zhao, Z. A. Xu, and S. J. Dong, S. J. Biosens. Bioelectron. 21, 984-988(2005).
10. K. Yamamoto, G. Y. Shi, T. S. Zhou, F. Xu, J. M. Xu, T. Kato, J. Y. Jin, and L.T. Jin, Analyst, 128, 249-254(2003).
11. W. J. Guan, Y. Li, Y. Q. Chen, X. B. Zhang, and G. Q. Hu, Biosens. Bioelectron. 21, 508-512(2005).
12. F. Patolsky, Y. Weizmann, and I Willner, Angew. Chem. Int. Ed. 43, 2113-2117(2004).
13. Y. J. Zhang, Y. F. Shen, J. H. Li, L. Niu, S. J. Dong, and A. Ivaska, Langmuir, 21, 4797-4800(2005).
14. M. Malmsten and A. Larsson, Colloid. Surf. B. Biointer. 18, 277-284(2000).
15. S. H. Choi and Y. C. Nho, Radiat. Phys. Chem., 58, 157-168(2000).
16. S. H. Choi, K. P. Lee and H. D. Kang, J. Appl. Polym. Sci., 88, 1153-1161(2003).
17. C. Gouveria-Caridade, R. Pauliukaite, and C. M. A. Brett, Electrochim. Acta, 53, 6732-6739(2008).
18. J. Wang, M. Musameh and Y. H. Lin, J. Am. Chem. Soc., 125, 2408-2409(2003).
19. H. Y. Gu, A.M. Yu, and H. Y. Chen, J. Electroanal. Chem., 516, 119-126(2001).
20. H. Feng, H. Wang, Y. Zhang, B. N. Yan, G. L. Shen, and R.Q. Yu, Anal. Sci., 23, 235-239(2007).
21. K. P. Lee, A. Gopalan, A. P. Santhosh, K. M Manesh, J. H. Kim, and K. S. Kim, J. Nanosci. Nanotechnol., 6, 1575-1583(2006).
22. Z. J. Wang, M. Li, P. P. Su, Y. Zhang, Y. F. Shen, D. X. Han, A. Ivaska, and L. Niu, Electrochem. Commun., 10, 306-310(2008).
23. L. T. Qu, L. Dai, and F. Osawa, J. Am. Chem. Soc., 128, 5523-5532(2006).
24. J. B. Jia, B. Q. Wang, A. G. Wu, G. J. Cheng, Z. Li and S. J. Dong, Anal. Chem., 74, 2217-2223(2002).
25. T. H. Li, H. G. Park and S. H. Choi, Mater. Chem. Phys., 105, 325-330(2007).
26. B. V. Enüstün and J. Turkevich, J. Am. Chem. Soc., 85, 3317-3328(1963).
27. N. Li, R.Yuan, Y. Q. Chai, S. H. Chen, H. Z. An, and W. J. Li, J. Phys. Chem. C, 111, 8443-8450(2007).
28. M. H. Piao, D. S. Yang, K. R. Yoon, S. H. Lee and S. H. Choi, Sensors, 9, 1662-1667(2009).
29. H. J. Kim, S. H. Choi, S. H. Oh, J. C. Woo, and I. K. Kim, J. Nanosci. Nanotechnol., 8, 1-6(2008).