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- E-ISSN 2288-8985
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Study on the immobilization of plant glutathione S-transferase for development of herbicide detection kit
Analytical Science and Technology / Analytical Science and Technology, (P)1225-0163; (E)2288-8985
2010, v.23 no.2, pp.172-178
https://doi.org/10.5806/AST.2010.23.2.172
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(2010). Study on the immobilization of plant glutathione S-transferase for development of herbicide detection kit. Analytical Science and Technology, 23(2), 172-178, https://doi.org/10.5806/AST.2010.23.2.172
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Abstract
Glutathione S-transferase is known to play a crucial role in detoxification in many cases. To develop a herbicide detection biosensor, we in this study attempted to immobilize glutathione S-transferase enzyme on solid supports, polystyrene and agarose, and Na-alginate. These matrixes were attractive materials for the construction of biosensors and might also have utility for the production of immobilized enzyme bioreactors. We also compared the activities of glutathione-S-transferase immobilized OsGSTF3 and free OsGSTF3. The specific activity of the free enzyme in solution was 3.3 higher than the immobilized enzyme. These results suggest that 50% of the enzyme was bound with the catalytic site in polystyrene-alkylamine bead and immobilized enzymes showed 80% remaining activity until 3 times reuse.
- keywords
- biosensor, glutathione S-transferase, herbicide detoxification, protein immobilization
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