- P-ISSN 1225-0163
- E-ISSN 2288-8985
Glucose oxidase (GODox) immobilized biosensor was fabricated by two methods. In one of the methods, gold nanoparticles (Au-NPs) prepared by γ-irradiation were loaded into the poly(maleic anhydride)-grafted multi-walled carbon nanotube, PMAn-g-MWCNT electrode via physical entrapment. In the other method,the Au-NPs were prepared by electrochemical reduction of Au ions on the surface of PMAn-g-MWCNT electrode and then GODox was immobilized into the Au-NPs. The GODox immobilized biosensors were tested for electrocatalytic activities to sense glucose. The sensing range of the biosensor based on the Au-NPs physically modified PMAn-g-MWCNT electrode was from 30 μM to 100 μM for the glucose concentration, and the detection limit was 15 μM. Interferences of ascorbic acid and uric acid were below 7.6%. The physically Au deposited PMAn-g-MWCNT paste electrodes appear to be good sensor in detecting glucose.
1. Z. Zhuang, X. Su, H. Yuan, Q. Sun, D. Xiao, and M.M.F. Choi, Analyst, 133, 126-132(2008).
2. F. Kurniawan, V. Tsakova, and V.M. Mirsky, Electroanalysis, 18, 1937-1942(2006).
3. L. Q. Rong, C. Yang, Q. Y. Qian, and X. H. Xia, Talanta, 72, 819-824(2007).
4. J. S. Ye, Y. Wen, W. D. Zhang, L. M. Gan, G. Q. Xu, and F. S. Sheu, Electrochem. Comm., 6, 66-70(2004).
5. C. K. Tan, K. P. Loh, and T. T. L. John, Analyst, 133, 448-451(2008).
6. S. Chakraborty and C. R. Raj, J. Electroanal. Chem., 609, 155-162(2007).
7. K. B. Male, S. Hrapovic, and J. H. T. Luong, Analyst, 132, 1254-1261(2007).
8. A. Arvinte, A. M. Sesay, V. Virtanen, and C. Bala, Electroanalysis, 20, 2355-2362(2008).
9. Y. Liu, M. K. Wang, F. Zhao, Z. A. Xu, and S. J. Dong, S. J. Biosens. Bioelectron. 21, 984-988(2005).
10. K. Yamamoto, G. Y. Shi, T. S. Zhou, F. Xu, J. M. Xu, T. Kato, J. Y. Jin, and L.T. Jin, Analyst, 128, 249-254(2003).
11. W. J. Guan, Y. Li, Y. Q. Chen, X. B. Zhang, and G. Q. Hu, Biosens. Bioelectron. 21, 508-512(2005).
12. F. Patolsky, Y. Weizmann, and I Willner, Angew. Chem. Int. Ed. 43, 2113-2117(2004).
13. Y. J. Zhang, Y. F. Shen, J. H. Li, L. Niu, S. J. Dong, and A. Ivaska, Langmuir, 21, 4797-4800(2005).
14. M. Malmsten and A. Larsson, Colloid. Surf. B. Biointer. 18, 277-284(2000).
15. S. H. Choi and Y. C. Nho, Radiat. Phys. Chem., 58, 157-168(2000).
16. S. H. Choi, K. P. Lee and H. D. Kang, J. Appl. Polym. Sci., 88, 1153-1161(2003).
17. C. Gouveria-Caridade, R. Pauliukaite, and C. M. A. Brett, Electrochim. Acta, 53, 6732-6739(2008).
18. J. Wang, M. Musameh and Y. H. Lin, J. Am. Chem. Soc., 125, 2408-2409(2003).
19. H. Y. Gu, A.M. Yu, and H. Y. Chen, J. Electroanal. Chem., 516, 119-126(2001).
20. H. Feng, H. Wang, Y. Zhang, B. N. Yan, G. L. Shen, and R.Q. Yu, Anal. Sci., 23, 235-239(2007).
21. K. P. Lee, A. Gopalan, A. P. Santhosh, K. M Manesh, J. H. Kim, and K. S. Kim, J. Nanosci. Nanotechnol., 6, 1575-1583(2006).
22. Z. J. Wang, M. Li, P. P. Su, Y. Zhang, Y. F. Shen, D. X. Han, A. Ivaska, and L. Niu, Electrochem. Commun., 10, 306-310(2008).
23. L. T. Qu, L. Dai, and F. Osawa, J. Am. Chem. Soc., 128, 5523-5532(2006).
24. J. B. Jia, B. Q. Wang, A. G. Wu, G. J. Cheng, Z. Li and S. J. Dong, Anal. Chem., 74, 2217-2223(2002).
25. T. H. Li, H. G. Park and S. H. Choi, Mater. Chem. Phys., 105, 325-330(2007).
26. B. V. Enüstün and J. Turkevich, J. Am. Chem. Soc., 85, 3317-3328(1963).
27. N. Li, R.Yuan, Y. Q. Chai, S. H. Chen, H. Z. An, and W. J. Li, J. Phys. Chem. C, 111, 8443-8450(2007).
28. M. H. Piao, D. S. Yang, K. R. Yoon, S. H. Lee and S. H. Choi, Sensors, 9, 1662-1667(2009).
29. H. J. Kim, S. H. Choi, S. H. Oh, J. C. Woo, and I. K. Kim, J. Nanosci. Nanotechnol., 8, 1-6(2008).