• P-ISSN1225-0163
  • E-ISSN2288-8985
  • SCOPUS, ESCI, KCI

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  • P-ISSN 1225-0163
  • E-ISSN 2288-8985

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    Sensitive and Mild Fluorogenic Reagents for Biogenic Carboxylic Acids in HPLC

    Analytical Science and Technology / Analytical Science and Technology, (P)1225-0163; (E)2288-8985
    1995, v.8 no.4, pp.545-551
    Ushijima, Tamano
    Saito, Mikihiko
    Sasamoto, Kazumi
    Ohkura, Yosuke
    Ueno, Keiyu
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    Abstract

    Five acid hydrazides as precolumn fluorescence derivatization reagents for carboxylic acids in HPLC, which have the benzofuran or benzothiazole moiety conjugated to a furan, thiophene or oxazoline ring, were synthesized and examined in view of reactivity, separability and sensitivity. Of these hydrazides, 2-(5-hydrazinocarbonyl-2-oxazolyl)-5,6-dimethoxybenzothiazole (BTOH) was most favorable. The detection limit of lauric acid as a model acid was 0.1 pmol per <TEX>$10-{\mu}l$</TEX> injection volume at S/N

    keywords
    High performance liquid chromatography, Acid hydrazide Fluorcscence derivatization reagents, Biogenic carboxylic acids


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