Sensitive and Mild Fluorogenic Reagents for Biogenic Carboxylic Acids in HPLC
Analytical Science and Technology / Analytical Science and Technology, (P)1225-0163; (E)2288-8985
1995, v.8 no.4, pp.545-551
Ushijima, Tamano
Saito, Mikihiko
Sasamoto, Kazumi
Ohkura, Yosuke
Ueno, Keiyu
Ushijima,
T., Saito,
M., Sasamoto,
K., Ohkura,
Y., &
Ueno,
K.
(1995). Sensitive and Mild Fluorogenic Reagents for Biogenic Carboxylic Acids in HPLC. , 8(4), 545-551.
Abstract
Five acid hydrazides as precolumn fluorescence derivatization reagents for carboxylic acids in HPLC, which have the benzofuran or benzothiazole moiety conjugated to a furan, thiophene or oxazoline ring, were synthesized and examined in view of reactivity, separability and sensitivity. Of these hydrazides, 2-(5-hydrazinocarbonyl-2-oxazolyl)-5,6-dimethoxybenzothiazole (BTOH) was most favorable. The detection limit of lauric acid as a model acid was 0.1 pmol per <TEX>$10-{\mu}l$</TEX> injection volume at S/N
- keywords
-
High performance liquid chromatography,
Acid hydrazide Fluorcscence derivatization reagents,
Biogenic carboxylic acids