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- E-ISSN 2288-8985
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- P-ISSN1225-0163
- E-ISSN2288-8985
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Enzyme Sensors Modified with Avidin/Biotin Systembased Protein Multilayers
Analytical Science and Technology / Analytical Science and Technology, (P)1225-0163; (E)2288-8985
1995, v.8 no.4, pp.591-596
Anzai,
J., Du,
X., Hoshi,
T., Suzuki,
Y., Takeshita,
H., &
Osa,
T.
(1995). Enzyme Sensors Modified with Avidin/Biotin Systembased Protein Multilayers. Analytical Science and Technology, 8(4), 591-596.
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Abstract
Enzyme multilayers composed of avidin and biotin-labeled enzymes were prepared on the surface of electrode, through a strong affinity between avidin and biotin (binding constant: ca <TEX>$10^{15} M^{-1}$</TEX>). The enzyme multilayers were useful for the improvement of the performance characteristies of enzyme sensors. The output current of the enzyme sensors depended linearly on the number of enzyme layers deposited. Thus, lactate oxidase (LOx) and alcohol oxidase (AlOx) were deposited after being modified with biotin for constructing enzyme sensors sensitive to L-lactate and ethanol respectively. It was also possible to deposit two different kinds of enzymes successively in a single multilayer. The glucose oxidase (GOx) and ascorbate oxidase (AsOx) were built into a multilayer structure on a Platinum electrode. The GOx, AsOx multilayer-modified electrode was useful for the elimination of ascorbic acid interference of the glucose sensor.
- keywords
- Enzyme multilayer, Enzyme sensor, Avidin-biotin complexation, Glucose oxrdase, Lactate oxidase, Alcohol oxidase, Ascorbate oxidase
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