This study examines the web-based education which is changing the educational paradigm itself according to the development of IT and the spread of Internet in the IT society and the information literacy which have been emerging as survival techniques. Based on Technology Acceptance Model that provides useful theoretical foundations for analyzing the factors influencing acceptance IT, it determined the factors influencing of accepting the information literacy instruction as follows: perceived usefulness; perceived ease of use; connectivity; reactivity of web-based education; job relevance; university library' support; ACRL standards and effects of the information literacy instruction. This paper confirmed the influences of these factors of accepting the web-based information literacy instruction through empirical study. I have suggested factors affecting the acceptance of web-based information literacy instruction by conducting positive researches on how these factors influence the acceptance of web-based information literacy instruction of university libraries.