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ISSN : 1226-9654
Effects of Electrolytic Lesion of the Prelimbic Area on Context-dependent Fear Discrimination in Rats
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Abstract
Recent studies suggest that the medial prefrontal cortex (mPFC) is necessary for the contextual modulation of fear memory. This study investigated whether the prelimbic (PL) subregion of mPFC has influence on the regulation of fear response to the ambiguous CS using contextual information. To verify the hypothetical role of PL, we adopted a context-dependent discriminatory fear conditioning where context dictates CS-US contingency. Rats received 3 pairings of the CS (30-sec, 4-kHz, 75-dB tone) and the US (0.5-sec, 0.5-mA footshock) in context A followed after 1 hour by 10 CS-only trials in context B for 4 days. In the last training session, all rats showed significantly more freezing in context A than in context B. Twenty-four hours after the last training session, PL was lesioned in half of the rats. After eleven days of recovery, we tested the effects of electrolytic lesion of PL on context-dependent discriminatory fear conditioning. PL-lesioned animals showed similar responding to the CS in context A and B, whereas sham-lesioned rats show significantly different responding. This result indicates that the lesion of PL disturbed discriminatory responses to the ambiguous CS when contextual information should be used to resolve the ambiguity of the CS. When all animals were subjected to subsequent training sessions (retraining sessions) for two additional days, PL-lesioned rats acquired context-dependent discriminatory fear memory during the retraining sessions. However, when the rats were tested on the next day, PL-lesioned animals did not show the differential responding to the CS in contextual A and B. This result indicates that PL is necessary for retrieving the learned discriminatory fear memory in the appropriate context. Taken together, these findings suggest that PL might be involved in fear expression under the conditions where the fear responses must be regulated by contexts.
- keywords
- medial prefrontal cortex(mPFC), prelimbic area(PL), context, fear discrimination, freezing, 내측전전두피질, 변연전영역, 맥락, 공포변별학습, 동결반응
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