This survey research examined the effectiveness, gender identity and the satisfaction of life of female leaders in government, in relation to the organizational culture regarding gender discrimination. The respondents were high -ranking (above 5th grade) male (N=154) and female (N=150) leaders in government and the supervisors (N=139) of the female leaders. The results demonstrated that the effectiveness of female leaders was rated lower as she helped her junior females at work more as a mentor, while she was rated as being more effective as her leadership style became more relationship-oriented. The satisfaction of life of female leaders was higher as she engaged more in mentoring to the junior females and as she had more positive gender identity. However, life satisfaction became lower as the organizational culture became more discriminating toward females. The discriminating organizational culture was also correlated negatively with the interpersonal relationship between females(especially the relationship with the female seniors). These results are indicating that the behaviors, the effectiveness ratings and the satisfaction of life of female leaders in Government are restricted by the gender -discriminating organizational culture and the cultural stereotypes of the members in the organization.