ISSN : 1226-9654
Data from our laboratory indicate that application of the mu agonist DAMGO into the basolateral complex of amygdala (BLA) suppresses tail flick reflexes in anesthetized rats. This DAMGO-induced antinociception can be blocked by pretreatment of the BLA with the nonselective opioid antagonist naltrexone, the mu opioid antagonist β-FNA, or the G protein inhibitor pertussis toxin, suggesting that DAMGO's interaction with G protein-coupled mu receptors in the BLA leads to production of antinociception. The present study employing the gene control strategy was conducted to further investigate the direct action of DAMGO on the mu receptors in the BLA. Intra-BLA application of antisense oligodeoxynucleotides (ODN) against mu receptors blocked antinociception following intra-BLA injection of DAMGO. The amount of [3H]-DAMGO binding to mu receptors in the amygdala was also reduced in the antisense ODN-pretreated rats. These data confirm the idea that antinociception induced by DAMGO in the BLA results from a direct interaction of DAMGO with mu receptors in the amygdala.
Adams, J. U., (1996) Functional effects of antisense oligodeoxynucleotides to opioid receptors in rats In: Antisense strategies for the study of receptor mechanisms, Antisense strategies for the study of receptor mechanisms,ed.by RB Raffa and F Porreca,pp.37-52,R.G.Landes Company.?
Atweh, S. F., (1977) Autoradiographic localization of opiate receptors in rat brain III, The telencephalon.Brain Research
Basbaum, A. I., (1978) Endogenous pain control mechanisms: Review and hypothesis, Annals of Neurology
Basbaum, A. I., (1984) Endogenous pain control systems: Brainstem spinal pathways and endorphin circuitry, Annual Review of Neuroscience
Bellgowan, P. S., (1998) The role of mu and kappa opioid receptors within the periaqueductal gray in the expression of conditional hypoalgesia, Brain Research
Bennet, G. J., (1979) Inhibition of spinal cord interneurons by narcotic microinjection and focal electrical stimulation in the periaqueductal gray matter, Brain Research
Bunzow, R., (1995) Characterization and distribution of a cloned rat mu opioid receptor, Journal of Neurochemistry
Carstens, E., (1995) Midbrain suppression of limb withdrawal and tail flick reflexes in the rat: Correlates with descending inhibition of sacral spinal neurons, Journal of Neurophysiology
Chen, Y., (1993) Molecular cloning and functional expression of a mu-opioid receptor from the rat brain, Molecular Pharmacology
De Olmos, J., (1985) Amygdala In: The rat nervous system: I. Forebrain and midbrain, Academic Press
Emmerson, P. J., (1994) Binding affinity and selectivity of opioids at mu, delta and kappa receptors in monkey brain membrane, Journal of Pharmacology & Experimental Therapeutics
Foo, H., (2000) Expression of antinociception in response to a signal for shock is blocked after selective downregulation of mu-opioid receptors in the rostral ventromedial medulla, Molecular Brain Research
Helmstetter, F. J., (1995) Microinfusion of mu but not delta or kappa agonists into the basolateral amygdala results in inhibition of the tail flick reflex in pentobarbital-anesthetizedrats, Journal of Pharmacology & Experimental Therapeutics
Helmstetter, F. J., (1993) Inhibition of the tail flick reflex following microinjections of morphine into the amygdala, NeuroReport
Helmstetter, F. J., (1998) Antinociception following?opioid stimulation of the basolateral amygdala is expressed through the periaqueductal gray and rostral ventromedial medulla, Brain Research
Heyman, J. S., (1988) Dissociation of opioid antinociception and central gastrointestinal propulsion in the mouse: Studies with naloxonazine, Journal of Pharmacology & Experimental Therapeutics
Hopkins, D. A., (1978) Amygdaloid projections to the mesencephalon, pons and medulla oblongata in the cat, Experimental Brain Research
Kalivas, P. W., (1982) Antinociception after microinjection of neurotensin into the central amygdaloid nucleus of the rat, Brain Research
Liu-Chen, L.-Y., (1987) Covalent labeling of m opioid binding site by [3H]- β-funaltrexamine, Molecular Pharmacology
Liu-Chen, L.-Y., (1995) Effect of intracerebroventricular β-funaltrexamine on m opioid receptors in the rat brain: consideration of binding condition, Journal of Pharmacology & Experimental Therapeutics
Mansour, A., (1994) Mu-opioid receptor mRNA expression in the rat CNS: comparison to mreceptor binding, Brain Research
Mansour, A., (1987) Autoradiographic differentiation of mu, delta and kappa opioid receptors in the rat forebrain and midbrain, Journal of Neuroscience
Mayer, D. J., (1974) Pain reduction by focal electrical stimulation of the brain: an anatomical and behavioral analysis, Brain Research
McGaraughty, S., (2004) Lesions of the periaqueductal gray disrupt input to the rostral ventromedial medulla following microinjections of morphine into the medial or basolateral nuclei of the amygdala, Brain Research
Morgan, M. M., (1991) Periaqeductal gray stimulation produces a spinally mediated, opioid antinociception for the inflamed hindpaw of the rat, Brain Research
Nandigama, P., (2003) Affective analgesia following the administration of morphine into the amygdala of rats, Brain Research
Nishino,K.,Su,Y.F.,Wong,C.-S.,Watkins,W.D.,& Chang,K.-J, (1990) Dissociation of mu opioid tolerance from receptor down-regulation in rat spinalcord, Journal of Pharmacology & Experimental Therapeutics
Oliveira, M. A., (2001) Role of PAG in the antinociception evoked from the medial or central amygdala in rats, Brain Research Bulletin
Pan, Z. Z., (1997) Cellular mechanism for anti-analgesic action of agonists of the k-opioid receptor, Nature
Pan, Y. X., (2000) Isolation and expression of a novel alternatively spliced mu opioid receptor isoform, MOR-1F, FEBS Letters
Pasternak, D. A., (2004) Identification of three alternatively spliced variants of the rat mu opioid receptor gene: dissociation of affinity and efficacy, Journal of Neurochemistry
Pasternak, G. W., (1995) Mapping of opioid receptors using antisense oligodeoxynucleotides: correlating their molecular biology and pharmacology, Trends in Pharmacological Sciences
Paul, D., (1989) Different mu receptor subtypes mediate spinal and supraspinal analgesia in mice, European Journal of Pharmacology
Paxinos, G., (1998) The rat brain in streotaxic coordinates, Academic Press
Rizvi, T. A., (1991) Connections between the central nucleus of the amygdala and the midbrain periaqueductal gray: Topography and reciprocity, Journal of Comparative Neurology
Rossi, G. C., (1997) Antisense mapping of MOR-1 in rats: Distinguishing between morphine and morphine-6β-glucuronide antinociception, Journal of Pharmacology & Experimental Therapeutics
Rossi, G. C., (1995) Antisense mapping the MOR-1 opioid receptor: evidence for alternative splicing and a novel morphine-6b-glucoronide receptor, FEBS Letters
Sancez-Blazquez, P., (1999) Antisense oligodeoxynucleotides targeting distinct exons of the cloned mu-opioid receptor distinguish between endomorphin-1 and morphine supraspinal antinociception in mice, Antisense Nucleic Acid & Drug Development
Schlingensiepen, R., (1997) Technical aspects of antisense oligonucleotides: In Antisensefrom technology to therapy: lab manual and text, Blackwell Science
Shin,M.-S., (2005) Vasoactive intestinal peptide in the amygdala inhibits tail flick reflexes in rats, Brain Research
Shin, M.-S., (2000) Pretreatment of the central, but not the basolateral, amygdala with muscimol blocks induction of mu-related antinociception following application of DAMGO, Society for Neuroscience Abstracts
Shin, M.-S., (2005) Antinociception following application of DAMGO to the basolateral amygdala results from a direct interaction of DAMGO with mu opioid receptors in the amygdala, Brain Research
Swajkoski, A. R., (1981) Blockade by naltrexone of analgesia produced by stimulation of the dorsal raphe nucleus, Pharmacology Biochemistry & Behavior
Tam, S. W., (1986) Reversible and irreversible binding of beta- funaltrexamine to mu, delta and kappa opioid receptors in guinea pig brain, Journal of Pharmacology & Experimental Therapeutics
Terashvili, M., (2005) Differential mechanisms of antianalgesia induced by edomorphin-1 and endormorphin-2 in the ventral periaqueductal gray of the rat, Journal of Pharmacology & Experimental Therapeutics
Tershner, S. A., (2000) Antinociception produced by mu opioid receptor activation in the amygdala is partially dependent on activation of mu opioid and neurotensin receptors in the ventral periaqueductal gray, Brain Research
Thompson, R. C., (1993) Cloning and pharmacological characterization of a rat mu opioid receptor, Neuron
Wahlestedt,C., (1994) Antisense oligodeoxynucleotide strategies in neuropharmacology, Trends in Pharmacological sciences
Wong, C.-S., (1992) Continuous intrathecal opioid treatment abolishes the regulatory effects of magnesium and guanine nucleotides on mu opioid receptor binding in rat spinal membranes, Journal of Pharmacology & Experimental Therapeutics
Young, R. F., (1987) Pain relief by electrical stimulation of the periaqueductal and periventricular gray matter, Journal of Neurosurgery