The aim of this study was to develop and validate the Multiphasic Anger Inventory (MAI), designed to measure state anger, trait anger, intimate state, tolerant trait, and negative growth experience. In Study 1, we examined the psychometric properties of the MAI in an adult sample nationwide (N = 926). Exploratory factor analyses and item-total correlations yielded a reliable and valid scale: state anger (10 items), trait anger (10 items), intimate state (4 items), and tolerant trait (4 items). In Study 2 (N = 407), we included negative growth experience (6 items) in addition to previously validated 28 items (state anger, trait anger, intimate state, tolerant trait) in the scale. We examined the reliability and validity of the MAI, employing exploratory factor analyses, confirmatory factor analyses, concurrent validity, internal consistency, and test-retest reliability. Overall, results provided support for the reliability and validity of the MAI, suggesting that the MAI is a useful instrument in the area of health psychology, clinical psychology, counseling psychology, organizational psychology, medicine, nursing, education, coaching, and criminal justice, among others.