The present study was to investigate job burnout in regards with diabetes that is a fast growing disease in adults over the world. Using data from healthy employees and employees with diabetes, the study conceptualized the health-work conflict that explains conflicts between the private role of illness management and the public demands from work. The study also investigated distress in diabetes as a local stressor of employees with diabetes and examined the effects of both the conflict and the distress on job burnout. Results revealed that the health-work conflict was highly reliable concept although there was a slight difference between healthy employees and diabetic employees. The conflict and distress were found to have close relationships with job burnout. Based on the findings, theoretical and practical implications were provided.