This study investigated whether skin conduction response(arousal) and gambling behavior(the amount of betting) is different in pathological gambling group(13) and control group(13) during the positive reinforcement(win) as well as punishment(loss). 594 undergraduates completed the K-NODS and impulsivity questionnaire(BIS-11). 594 undergraduates asked to complete K-NODS and BIS-11. They were divided two group according to th severity of gambling, the experimental subjects(N=26) were selected. 13 subjects were of pathological gambling group, 13 subjects were of normal group. In the experimental study, The two groups were administrated a slot machine game. Skin conduction response as the index of arousal and the amount of betting as gambling behavior was measured when participants played a slot machine. This Study was failed to find the differentiated effect of arousal and the amount of betting depending on gambling conditions. However, Pathological gambling group had higher SCR and the amount of betting compared to control group when was exposed to positive stimuli(win og gambling). Finally, limitations and suggestions of this study were discussed.