Noise influences not only hearing but also the brain. High density noise, as a stressor, leads to increased level of excretion in catecholamine. For this reason, the level of catecholamine has been used as an indicator to stress, Specially, unexpected and repeated noise increased the level of catecholamine in urine. The purpose of the present study was to examine the effect of noise on catecholamine level in a white rat's urine. The subjects were subjected to an exposure of 90 dB noise for a period of 10, 30, 60 minutes, and 3, 12,and 24 hours. When catecholamine level was assayed with HPLC-ECD, after extracting urine with alumina, it was found that noise influenced the catecholamine level.